Coding

Part:BBa_K1710000:Design

Designed by: Jacob A. Palumbo   Group: iGEM15_PacificU-Oregon   (2015-08-28)


Tcas phenoloxidase 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 278
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1220
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 278
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 278
    Illegal NgoMIV site found at 703
    Illegal NgoMIV site found at 1674
    Illegal NgoMIV site found at 1923
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequenced was codon optimized for E. Coli using IDT's codon optimization tool.


Source

This part is derived from the lac2 gene from the Tribolium castaneum genome.

http://www.ncbi.nlm.nih.gov/protein/193211474

References